【摘要】 本文采用辣根过氧化物酶(HRP)作为抗原,与福氏佐剂一起对雄性豚鼠进行二次免疫,追踪腘淋巴结中抗体形成细胞的发育和分化。在二次免疫后的早期(2—3天),淋巴结的皮质区(特别是淋巴滤泡间区)和髓质区几乎同时出现阳性反应的细胞,主要是淡棕色的小淋巴细胞和中淋巴细胞。至6—9天,这二个区域的阳性细胞数明显增加达到高峰,主要是棕色和棕褐色的前浆细胞和浆细胞,皮质区与髓质区的阳性细胞在某些区域相连接。从12天起这二个区域的阳性细胞开始下降,在27—49天,淋巴结中只存在少数分散的阳性细胞,部分细胞萎缩变形成残留的浆细胞。在同一切片视野中除了看到棕褐色浆细胞和淡棕色小淋巴细胞外,还可以看到包括中淋巴细胞和前浆细胞等过渡型细胞。因此,我们认为浆细胞是分别在皮质区和髓质区由淋巴细胞发育而成。更多还原

【Abstract】 The development of antibody-producingimmunocytes was examined in the popliteallymph nodes of guinea pigs during the secondary response to horseradish peroxidase(HRP).The cells containing antibody weresimultaneously observed in the medulla cordregion and in the interfollicular region of thecortex 2-3 days after the boosting.The po-sitively reactive cells were mainly small andintermediate lymphocytes and the specificstaining reaction of anti-HRP antibody inthese cells was weak.Besides these cells afew proplasma cells with an enlarged nucleiand cell size and intermediate staining inten-sities were observed in these regions. A remarkable increase of antibody posi-tive cells,including proplasma cells and plas-ma cells,were observed in lymph nodes bet-ween the 3 rd—9 th days after a boost ofHRP.The mature plasma cells manifested afurther increase of cytoplasm and a decreaseof nucleus/cytoplasm ratio.The positive cellsin medulla were in close contact with thepositive cells in cortex in some regions. From the 12 th day to 49 th day afterthe boosting,the number of antibody-produ-cing cells decreased substantially.It was ob-served that small lymphocytes and plasmacells were accompanied sometimes by inter-mediate lymphocytes and proplasma cells inthe same microscopic visual field. It seems that plasma cells evolve fromsmall lymphocytes by separate lines of deve-lopment both in medulla and in cortex re-gions.更多还原