【摘要】 将人外周血富集的T淋巴细胞置于40—42℃(热休克)的环境中培养,以模拟机体发热时细胞所处的环境温度,通过标记氨基酸参入实验发现热休克至少可诱导十余种分子量不同的多肽合成,其中以71,000和90.000d分子量的多肽合成最为旺盛。本文还对主要热休克蛋白的诱导特异性和动力学方面的特点进行了研究和讨论。更多还原

【Abstract】 Enriched human peripheral T lymphocytes were exposed to elevated temperature of 40-42℃(heat shock) to mimic the circumstances encountered in vivo under fever.More than ten peptides with different apparent molecular mass have been induced during heat shock.The most predominant peptides synthesized were those with apparent Mr of 71 and 90K.Some minor ones,usually expressed after 3 hours of heat shock were also found.Kinectics of major heat shock protein (HSP) synthesis have been investigated with either different heat shock temperature or incorporating labeled amino acids during a prolonged period of heat shock up to 20 hours.更多还原