【摘要】 用DPH和ANS标记大鼠肝线粒体内膜,以稳态荧光偏振法,研究了呼吸链底物和抑制剂对内膜流动性的影响。1.苹果酸+谷氨酸、琥珀酸分别为底物,均能引起内膜流动性增加。2.琥珀酸对含心磷脂的脂质体的膜流动性无影响。3.在鱼藤酮存在的条件下,苹果酸+谷氨酸对内膜流动性的增加作用消失,但琥珀酸的作用仍然存在。有氰化钾时则琥珀酸的作用消失。4.不论外加底物存在与否,鱼藤酮使内膜的流动性下降,而氰化钾则使之增加。抗霉素A亦可使内膜的流动性增加。上述结果表明:线粒体内膜流动性与其功能密切相关。电子沿呼吸链传递使线粒体内膜流动性增加,这种变化可能与呼吸链成分的氧化还原态有关。更多还原

【Abstract】 The effects of both substrates and inhibitors for respiratory chain on membrane fluidity of mitochondrial inner membrane (mitoplast) were investigated with fluorescence polarization technique using DPH and ANS as probes.1.The membrane fluidity of mitoplast was increased by adding a substrate of malate plus glutamate,or succinate alone.2.No effect of succinate on membrane fluidity was observed for liposome containing cardiolipin.3.The presence of rotenone abolished the effect of malate plus glutamate on membrane fluidity,but not the effect of succinate.The effect of succinate was abolished by the presence of potassium cyanide.4.In systems with or without added substrate of malate plus glutamate the membrane fluidity of mitoplast was always decreased by the addition of rotenone,but increased by the action of potassium cyanide.Increased membrane fluidity resulted from the addition of antimycin A was also observed.These results indicated that membrane fluidity and function of the mitoplast were closely related.Initiation of electron transfer along respirtory chain resulted in an increase in membrane fluidity of mitoplast.The membrane fluidity was probably affected by the redox state of the components in respiratory chain.更多还原