【摘要】 用反相高效液相色谱（RP—HPLC）法,测定家兔和人血浆中醋酸甲地孕酮（MA）浓度,并由兔血药浓度-时间曲线算得药物动力学参数。 以醋酸炔诺酮为内标、乙酸乙酯提取血浆中MA,于YWG—G₁₈H₃₇柱上进行色谱测定。应用梯度洗脱法选择甲醇-水二元恒溶剂流动相,其组成预期值为MeOH-H₂O（73:27）。本法准确度与精密度良好,血浆中MA的提取回收率平均为85.86±6.16％;方法回收率平均为101.7±7.73％。最低检出浓度为16ng/ml血浆。实验表明,本法简便可行,可提供计划生育临床MA血浓度监测需要。更多还原

【Abstract】 A reversed phase high performance liquid ohromatographio method (RP-HPLC) for the determination of human and rabbit plasma levels of megestrol acetate (MA), an effective contraceptive drag, was developed; and its pharmacokinetic parameters were obtained from the rabbit plasma level-time curve measurements.MA in the plasma was determined by adding norethindrone acetate as an internal standard, extracting with ethyl acetate, chromatographing on a 15cm ×5mm I.D. column packed with YWG-C18H37(10Mμm), and detecting at 254nm. The mobile phase selected by gradient elution was methanol: water (73:27).The average extraction recovery of MA from plasma was 85.86±6.16%, CV = 7.17%; the average recovery of MA of the entire procedure, 101.7±7.73%, CV= 7.6%. The minimum detection limit was 16ng/ml plasma.更多还原