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用固相ELISA-C1q结合试验检测病毒性肝炎的循环免疫复合物
DETECTION OF CIRCULATING IMMUNE COMPLEXES IN VIRAL HEPATITIS USING A SOLID PHASE ELISA-C1q BINDING TEST
【摘要】 用改进的亲和层析法提取C1q,建立了固相酶联免疫吸附法（ELISA）C1q结合试验并检测各组病毒性肝炎患者的循环免疫复合物（CIC）,探讨了实验中若干影响因素。
【Abstract】 A solid phase ELISA-C1q binding test was developed for detection of circulating immune complexes (CIO) in viral hepatitis. The use of C1q purified by affinity chromatography and the appropriate conjugate in this system showed low background and high specificity. The sensitivity was as high as 0.5μg aggregated human IgG (AHG) equivalent/ml and the average concentration of CIC in 61 normal controls was 53.69μg AHG equivalent/ml. Some of the factors which could influence the test were discussed.
【关键词】 酶联免疫吸附法；
Clq结合试验；
循环免疫复合物；
病毒性肝炎；
【Key words】 enzyme-linked immunosorbent assay (ELISA)； C1q binding teat； circulating immune complexes； viral hepatitis；
【Key words】 enzyme-linked immunosorbent assay (ELISA)； C1q binding teat； circulating immune complexes； viral hepatitis；
- 【文献出处】 上海医科大学学报 , 编辑部邮箱 ,1986年06期
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