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用ABC方法研究两种小鼠白血病分型

TYPING STUDY OF TWO MODELS OF MICE LEUKEMIA BY THE ABC METHOD

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【作者】 许良中; 陈红莉; 张泰明;

【Author】 XU LIANGZHONG, CHEN HONGLI, ZHANG TAIMING(Laboratory of Pathology, Cancer Hospital, Shanghai Medical University, Shanghai)

【机构】 上海医科大学肿瘤医院病理学研究室; 上海医科大学肿瘤医院病理学研究室;

【摘要】 本文通过卵白素生物素复合物(简称ABC)免疫组织化学的方法标记Thy1、2,Lyt-1,Lyt-2和Iak~2四种单克隆抗体,研究了L7712和P388两种小鼠白血病模型的免疫学分型。实验结果提示L7712属于抑制性T淋巴细胞性白血病,P388属于B淋巴细胞性白血病。

【Abstract】 The immunohistoohemical characteristics of leukemia in mice were inve stigatd with monoclonal antibodies Thy1,2, Lyt-1, Lyt-2 , and Iak2 using the ABC immunological method. Results showed that all the tumor cells from L 7712 were strongly positive for Thyl, 2 and Lyt-2 and negative for Lyt-1 and Iak2 indicating their origin from suppressor T-lymphqcyte. All the tumor cells from P388 were strongly positive for Iak2 and negative for Thyl, 2, Lyt-1 and Lyt-2 indicating their origin from the B-lymphocyte and not T-lymphocyte. Other related data are discussed.

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