【摘要】 用直接法制备染色体、病理切片和放射自显影技术,观察3’-甲基-4,4’-二甲基偶氮苯（3’-Me-DAB,三甲基奶油黄）诱发大鼠肝癌的诱癌过程。发现诱癌过程中鼠肝组织内细胞增生活跃:早期为卵圆细胞和残留的肝细胞;中期为增生结节内的肝细胞及汇管区胆管上皮细胞;后期为肝癌细胞。同时也发生了染色体数目及形态的变化。染色体畸变以染色单体型为主,提示致癌剂作用于S和G₂期细胞。1、2号染色体断裂率高于理论值,这可能与染色体结构重排有关。癌变过程中染色体畸变可能有累积和放大。我们还对肝癌的发生与染色体畸变的关系进行了探讨。更多还原

【Abstract】 Chromosomal analyses, auto-radiography, and routine pathological techniques, were used in the study of hepatocarcinogenesis in rats. It was found that rat liver cells proliferated actively during the precancerous and the cancerous stages with simultaneous occurrence of chromosomal aberrations especially numerically. The appearance of structural aberrations (chromatid type) suggested that some cells were just in stages S and G2 when they were affected by 3’-Me-DAB. In some chromosomes (1*, 2*) the incidences of breaks and gaps were high. There was evidence that some cells went through more than one cell cycle, indicating that chromosomal changes might be cumulative with amplification of effects. Based upon experimental results, the relationship between hepatocarcinogenesis and chromosomal aberration was discussed.更多还原