【摘要】 用聚丙烯酰胺凝胶电泳(PAGE)分离纯化大鼠羊水中的甲胎蛋白(AFP)具有简便、省时的特点。将微量AFP注入淋巴结内,制备高效价的AFP抗体。比较四种固定液对AFP免疫组织化学染色的影响,示丙酮-甲醛固定液能很好保存AFP的抗原性,具有阳性颗粒强、背景染色淡的优点,故是AFP免疫组织化学的最佳固定液。更多还原

【Abstract】 Separation and purification of α-fetoprotein(AFP)in rats and preparation of its’ antibody are the necessary processes for studying hepatocarcinogenesis. It is not only simple but also saves time to separate antigens by preparative polyacrylamide gel electrophoresis. Using this method, AFP was successfully separated from the amniotic fluid of rats. Titer of anti-AFP antibody was raised by injecting of micro-amount of AFP into the lymph nodes in rabbits. Comparing the effects of four kinds of fixatives,i. e. acetone-formaldehyde, periodate-lysine-paraformaldehyde(PLP),picric acid-paraformaldehyde, and 95% alcohol, on immunohistochemical staining for AFP in rat liver, it was considered that acetone-formaldehyde fixation gave best preservation of the antigenicity of AFP and had the advantages of stronger staining and weaker background color, so it was the best fixative for the staining of AFP in immunohistochemistry.更多还原