【摘要】 从牛脑和人脑内分别提取神经元特异性烯醇酶(Neuron-specific enolase,简称NSE,又称γγ烯醇酶),并制备了兔抗牛NSE抗血清(抗NSE_b)和兔抗人NSE抗血清(抗NSE_h)。用猫、狗的脊髓和大脑运动皮质切片,进行免疫组织化学-PAP法染色,其中神经元均呈阳性反应,神经胶质均阴性,并与美国的兔抗人NSE抗血清(抗NSE_H)进行染色对比。更多还原

【Abstract】 Neuron-specific enolase (NSE) was eluted from the human (NSEh) and bovine (NSEb) brain, and anti-NSEh and anti-NSEbsera were prepared by Grasso’s method.Immunohistological unlabeled antibody peroxidase antiperoxidase method was used to determine the distribution of NSE in the spinal cord and cerebral motor cortex of the domestic cat and dog. Positive reactions were found in all the neurons of spinal cord and cerebral motor cortex of both animals, but not in the glia. Concurrent experiments using rabbit antiseruiu from Polysciences Inc. USA, showed no difference in staining reactions.更多还原