【摘要】 单向火箭免疫电泳测定大鼠血清载脂蛋白AI(Apo AI)浓度,操作简单,定量准确。待测样品采用9M脲致变性使抗原决定簇暴露,减少了样品处理时间,得到较好的测定结果。用此法测得雄性和雌性SD大鼠血清Apo AI浓度分别为45.76±6.0mg/dl(n=10)、60.60±9.4mg/dl(n=10)。批内CV为4％,批间CV为7％。正常大鼠注射胰岛素,血清Apo AI浓度明显降低。更多还原

【Abstract】 Rocket electrophoresis measurement of rat serum apolipoprotein A-I (Apo A-I) concentration wasfound to be simple and accurate. Apo A-I was denatured and the antigenic determinant was exposed by treatment with 9 M urea. This treatment reduced assay time and the results were quite satisfactory. Concentrations of Apo A-I in male and female SD rats by rocket electrophoresis were 46.76±6.0mg/dl (n = 10) and 60.60±9.4mg/dl (n = 10) respectively. The intraassay coefficient of Variation was 4%. The interassay coefficient was 7%.When rats were treated with insulin, the serum Apo A-I concentrations decreased significantly.更多还原