【摘要】 本文研究了测定谷氨酸的微生物传感器。用琼脂固定化的大肠杆菌(Escherichia (?)oliAS 1.505)和二氧化碳电极制成膜状传感器,用聚丙烯酰胺固定化的大肠杆菌和二氧化碳电极制成柱状传感器。结果表明,膜状传感器在25℃测定谷氨酸的范围为60—1200mg/1,响应时间为1020min;柱状传感器在30℃测定谷氨酸的范围为60—800mg/1,响应时间为5—7min。两种传感器测定误差为2—3%以下,20天内使用60多次,不见其活力下降。更多还原

【Abstract】 The microbial sensor measuring glutamate has been studied.The systemof membrane microbial sensor is composed of Escherichia coli AS 1.505 im-mobilized by agar and CO₂ electrode.The system of bed microbial sensoris composed of Escherichia cali AS 1.505 immobilised by poliacrylamideand CO₂ electrode.It is shown that the area of membrane microbial sensor for measuringglutamate is 60—1200mg/liter at 25℃ and the respondent time is 10—18min.;the area of bed microbial sensor for measuring glutamate is 60—800mg/liter at 30℃ and the respondent time is 5—7 min.The error of theboth microbial sensor is less than 2—3% and after measuring 60 timesduring 20 days the activity of measuring glutamate is less decreased.更多还原