【摘要】 本文首次报道以家蚕(Bombyx mori Linnaeus)为材料,选择五龄幼虫期的黑缟品种的真皮组织及沔阳红品种的丝腺,分离出模板活性染色质,获得了预期的目的基因—Ps 及Pk。把它们用显微注射及激光微束技术分别转移到华合(白蚕)、龙角蚕、巴陵黄(黄茧)的受精卵中,观察到黑缟基因(P~s)、红茧基因(Pk)在白蚕(华合)、黄茧蚕(巴陵黄)中的当代表达及在子代中的传递情况。更多还原

【Abstract】 This paper is the first report of gene transfer by template-active chro-matin.We isolated the template-active chromatin from the silkglands ofMianyang red (red cocoon silkworm) and the Hypoderm of Heigao (blacksilkworm),and obtained the expectant genes (P~S).Tranferred them intoeggs of Baling yellow (yellow cocoon silkworm)、Dragon-hornsilkworm andHuahe(white silkworm) by microinjection(or laser microbeam sometimes)andthen some exogenous genes can be expressed and transmitted to progeny.This technique will allow us to transfer exogenous genes selectively in acircumstances which we don’t understand the gene sequence and its mRNAas yet.It may be a new approach of genetic engineering for eukaryote.更多还原