【摘要】 用由结肠癌肝转移灶纯化的CEA抗原免疫BALB/C小鼠,其脾细胞与鼠骨髓瘤细胞SP2/O融合,制备产生 CEAMcAb 的杂交瘤。用 ELISA 法检测与 CEA、NCA、NCA-2的反应特异性。获得8株仅与 CEA 有结合反应的 McAb,通过阻断抑制试验证明这8株 McAb认识 CEA 分子上三个不同的抗原决定簇。它们的免疫球蛋白亚类同是 IgG₁。更多还原

【Abstract】 Hybridomas producing monoclonal antibody to carcinoembryonic antigen（CEA）have been developed by fusion of mouse myeloma cell line SP 2/Owith splenocytes of BALB/C mice which are immunized with purified CEAfrom hepato-metastasis of colonic carcinoma.The ELISA method is usedin examining the specificity of the antibody with CEA,NCA and NCA-2.Eight monoclonal antibodies bounded only to CEA have been obtained fin-ally.These eight CEA specific antibodies have been proved to be recogni-zed three differential epitope on CEA molecular accoding to the blockinginhibition assay.The Ig subclasses of these CEA McAbs have been determi-ned to be the same IgG₁.更多还原