【摘要】 本文以酵母-大肠杆菌穿梭质粒YEP51为载体构建了酿酒酵母S.cerevisiae 2.1168的基因文库,并用 DNA 分子杂交技术从这个基因文库取得了包括 SUC2基因的克隆 YFD6。本文还对 YFD6中的 SUC2基因在酵母细胞中的表达、YFD6的物理图谱以及 SUC2基因在 YFD6上的位置作了初步分析。更多还原

【Abstract】 By using the yeast-E.colt shuttle vector YEP51,a gene library ofS.cerevisiae 2.1168 was constructed.By means of DNA hybridization techni-que a.SUC2 gene containing clone was fished out from the library.Theexpression,of SUC2 gene of YFD6 in yeast,the physical map of YFD6 andthe locatization of SUC2 gene on YFD6 were preliminarily studied.更多还原