【摘要】 本文研究了抗纯化N.Sertifer NPV的杂交瘤抗体。病毒的包涵体是通过K-系列离心而提纯,由Frank Lewis博士提供。每个BALB/C小白鼠每次用200μg抗原免疫。最后一次注射是在细胞融合之前4～7天。取2×10⁸脾细胞与2×10⁷骨髓瘤细胞（NS-1或P₃×63）在50％PEG 4000（Sigma）存在下进行融合,用HAT来选择杂交瘤细胞。用间接ELISA法来筛选阳性的杂交瘤细胞。 13个细胞系已筛选成功,并对其产生的免疫球蛋白进行了分型。选择三种Lepidopteran NPVs用于交叉保护试验。除三种杂交瘤抗体外,其他10种对这三种病毒均有不同程度的交叉反应。更多还原

【Abstract】 In this study, hybridoma antibodies to the purified Neodiprion sertifer nuclear polyhedrosis virus （European pine sawfly） were developed. Virus inclusion bodies, purified with the K-series centrifuge was supplied by Dr. Frank Lewis. Each BALB/c mouse was immunized with 200ug of antigen for each booster. The last booster was given 4-7 days prior to cells fusion. Pooled spleen cells （2×10⁸） are mixed with NS-1 or P₃×63 mycloma cells（2×10⁷） and tused with 50％ PEG 4000 （Sigma）. Hybridomas selected with HAT medium. The indirect ELISA method was used to screen for positive hybridomas. Thirteen cells lines were selected. The immunoglobulin subclass of the hybridoma antibodies were determined using the indirect method. Three epidopteran NPVs were chosen for the crossreactivity study. The result in fable 1 showed that most of the hybridoma antibodies reacted differentially with the lepidoptern virus antigens except from three clones. All these hybridoma antibodies will be useful as tools for biochemical studies of baculoviruses. The three clones could potentially be useful diagnostic reagents.更多还原