【摘要】 本文以马来酰亚胺自旋标记的人血红蛋白样品为例,阐述了饱和转移电子顺磁共振实验技术,其中包括样品制备、测量要点和波谱参数的测定。并探讨了实验条件对波谱的影响。更多还原

【Abstract】 A description of the experimental technique of saturation transfer electron paramagnetic resonance (ST-EPR) is given by using maleimide spin labeled hemoglobin as an example. It relates to the main points of the sample preparation and the experimental measurement as well as determination of the parameters of ST-EPR spectrum. In addition, the present paper goes further into the problems about the influences of the experimental conditions on ST-EPR spectra.更多还原