【摘要】 本文采用硷化乙醇法制备细菌O抗原,用以致敏醛化红细胞。临用前加入3%聚乙二醇(PEG)、灭活的正常兔血清及绵羊红细胞膜配成间接血凝应用液,以检测杂交瘤产生的抗霍乱弧菌菌体抗原的单克隆抗体。在细胞培养上清和小鼠腹水抗体的检测中,其灵敏度比经典的醛化致敏血球提高4～8倍。更多还原

【Abstract】 We used the base-alcohol method to extract bacterial O-antigen,with which the aldehyde-treated sheep red blood cells(SRBC)were sensitized.The applicable fluid for the passive hemagglutination test was prepared by adding 3% polyethylene glycol(PEG),the normal rabbit sera treated at 56℃ and SRBC membrane into the SRBC suspension.The fluid were used to detect monoclonal hybridoma antibodies against V.cholerae O-antigens.The sensitivity of the test was proved to be 4—8 times as sensitive as that of classical passive hemagglutination test.更多还原