【摘要】 本文介绍用酶联免疫吸附检测法(ELISA)检测牛白血病病毒(BLV)的抗体。以FLK-BLV细胞培养液制备的免疫扩散(ID)用的粗提抗原,经ConA-Sepharose 4B亲和层析,或先经乙醚处理,再经Sephadex G-150层析等方法,分别提取了gp和p抗原。用gp抗原作ELISA,对91份牛血清样品进行检测,阳性共67份,比微量免疫扩散(MID)高14％,两者的符合率为85％。用p抗原作ELISA对240份牛血清样品进行检测,阳性占162份,比MID法高12％,两者的符合率为87％。用gP抗原对33份样品和用p抗原对160份样品进行三次重复试验,它们的变异系数在10％以内的分别为100％和92.5％。ELISA抑制试验表明,p抗原和gP抗原对BLV抗体的特异性良好。更多还原

【Abstract】 The report described the enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to bovine leukemia virus (BLV). The gp and pantigens of BLV were extracted separately from crude antigen which was prepared from FLK-BLV cell culture fluid and used in immunodiffusion test. The crude antigen flowed through Con A-Sepharose 4B affinity chromatogra phic column, and the concentrated eluent was gp antigen. If the crude antigen was treated with ethyl ether, followed by chromatography of Sephadex G-150, the eluent was p antigen. Among 91 samples of cow sera examined by ELISA with gp antigen 69 positives were detected, which was 14% higher than that of microimmunodiffusion (MID), and the agreement between two methods was 85%. When 240 samples were examined with p antigen, 162 positives were detected, which was 12% higher than that of MID, and the agreement was 87%. When 33 samples detected with gp antigen and 160 samples with p antigen were repeated three times, the variation coefficient below 10% was 100% and 92.5% respectively. The ELISA inhibition test showed that p and gp antigens to BLV antibodies were highly specific.更多还原