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应用热释光剂量测定法检测农作物活体叶片14C同化物输出的研究

MEASURING THE EXPORT OF 14CO2 ASSIMILATES IN TREATED LEAVES IN VIVO BY TLD METHOD

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【作者】 张荣铣; 陈祖义; 朱根海; 李培武; 朱培仁; 于凤义; 赵光辉;

【Author】 Zhang Rongxian, Chen Zuyi, Zhu Genhai, Li Peiwu, Zhu Peiren(Section of Plant Physiology, Nanjing Agricultural University)Yu Fengyi and Zhao Guanghui(Atomic Energy Research Institute, Chinese Academy of Agricultural Sciences)

【机构】 南京农业大学植物生理教研组; 南京农业大学同位素应用教研组; 中国农科院原子能研究所; 中国农科院原子能研究所;

【摘要】 在14CO2饲喂植物叶片后,以CaSO4(Dy)-TEFLON热释光片,贴于饲喂叶片上检测0.5至1小时,用圆产FJ-377热释光剂量仪(北京核仪器厂)读取TL值(热释光读数值),说明热释光片对14C释放的β能量具有一定响应性。使用FJ-377热释光剂量仪时,热释光片以φ5.5×0.4mm的检测效果优于φ8.0×0.5mm。热释光剂量测定法(TLD)的检测与液闪法测定比较,两者结果一致,TL值与dpm/cm2呈极显著相关(r=0.94,P<0.01)。取大豆、葡萄为材料,经多次连续检测TL值,观察TL值下降(或递增)百分率以估计14C同化物的输出与输入情况。实践证明,应用TLD较液闪法简便、快速,适于在田间活体植株上多点测试。

【Abstract】 There was a response of thermoluminecence dosimetry (TLD) discs to 14C radiation energy released from 14CO2 assimilates in leaves. The thermoluminescence dosimetry dises, CaSO4(Dy)-TEFLON discs,were prepared by Atomie Energy Research Institute, Chinese Academy of Agricultural Sciences. The discs were put on the blade for30 to 60minutes which was fed with 14CO2. The thermolumineeenee values (TL values) appeared with thermoluminescence measuring instrument (Model FJ-377, made in China). There were two types of TLD discs, φ5.5×0.4 and φ8.0×0.5mm. As to effect, the former is better than the latter. The results measured by TLD method showed no difference as compared with those measured by scintilation counter method. There was a high significant positive correlation between TL value and dpm/cm2, r=0.94, P<0.01. TLD was used to measure the TL value in soybean and grape leaves at regullar intervals, then to calculate their thermolumineseence attenuation rate and increasing rate. These were good indies to express transportation of 14C assimilates (import or export).Clearly, TLD is a simple, rapid and widely used method. It is suitable for measuring many samples with intacted leaves in vivo both in the laboratory and in the field.

  • 【文献出处】 南京农业大学学报 ,Journal of Nanjing Agricultural University , 编辑部邮箱 ,1986年02期
  • 【被引频次】4
  • 【下载频次】39
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