【摘要】 研究表明,在整个韭菜叶肉细胞的发育过程中,其原生质体的体积将扩大约30倍,它们的最小的原生质体直径约15微米,而最大的原生质体直径可达60微米。用扫描电子显微镜和暗相差显微镜观察发现,发育早期叶肉细胞的原生质体的质膜柔软带有很多褶皱和密集的颗粒结构,适于细胞的迅速生长和扩大,对细胞松驰素B敏感。细胞松驰素B能诱导原生质体融合,排核和产生微原生质体。同时对质膜有损伤,并产生疤状结构。相反发育成熟的叶肉细胞,由于不继续生长和扩大,原生质膜变得平滑,对细胞松驰素B的作用不敏感,不能或很少引起原生质膜融合.更多还原

【Abstract】 This study reveals that volume of mesophyll protoplast of Chinese chives expands about 30-fold in all the developmental period of mesophyll. The smallest protoplast is about 15μand the Largest one is about 60μ in diameter. Scanning electron microscope and dark phase contrast microscope observations show that the protoplasmic membrane of protoplast of early developmental mesophyll is soft with many folds and dense grain structure. Cytochasin is able to induce protoplast fusion and extrusion of nucleus and produce microportoplasts,but, at the same time the protoplasmic membrane of protoplast is injured with belb structure.On the contrary,as mature mesophyll will not grow and enlarge, or rarely enlarge, the protoplasmic membrane of its protoplast become flat and smooth and is not sensitive to the action of cytochalasin B and hardly induce protoplasmic membrane fusion.更多还原