【摘要】 从表现退绿晕斑、皱缩变形(P-Ⅰ)和卷叶、脉间黄化(P-Ⅱ)系统花叶症状的泡桐嫩叶上分离到两种病毒颗粒。根据两种分离物鉴别寄主反应,电镜下病毒形态及血清学等特点,确定P-Ⅰ属黄瓜花叶病毒组的黄瓜花叶病毒;P-Ⅱ属烟草花叶病毒组的烟草花叶病毒,并分别记作CMV-Pa和TMV-Pa。更多还原

【Abstract】 Leaves with systemic symptoms of ring or irregular spots and deformation (P-Ⅰ), and rolling leaves with yellowing inter-vein (P-Ⅱ), were collected from Hainan province and Beijing. The infected young leaves were triturated in a mortar with a pestle in 0.01M sodium phosphate buffer(pH7.2) containing antioxidate of 0.1％ diethyldithioca-rbamate. The sap can infect both Nicotiana tobacum and N. tobacum cv. Xanthi-nc. And other herbaceous plants could also be infected with the two bridge hosts by mechnical transmission.Based on the morphology of the particles, serological reactions, physical properties and symptoms on differential hosts, P-Ⅰ and P-Ⅱ were considered to be CMV of Cucumoviruses and TMV of Tobamoviruses, signed by the symbol CMV-Pa and TMV-Pa, respectively.更多还原