In order to explore the application of anther culture to hybrid breeding and the purification and rejuvenation of the three lines of Oryza sativa subsp. shien, we have studied systematically the following aspects since 1980.
In anther culture of hybrid rice using the He-5 medium, results showed that anthers from different donors varied considerably in their ability to produce pollen-calluses and plantlets. The average frequency of callus induction was 3.66% for the hybrid rice involving wild-aborti...