【摘要】 鱼卵透明带热溶法分离糖蛋白组分,按常规法取这种粗品0.1～0.2毫克,加福氏佐剂乳化肌注,三次强化后即可使抗体滴度达2⁴,五周后高达2^?.主动免疫小白鼠卵巢及血清中,雌二醇和孕酮较对照组偏低,生殖周期有一定延长,但组织学研究未发现主动免疫小白鼠卵泡有任何异常变化,卵泡生长、发育、成熟及排卵活动正常,排卵数与正常对照组相近似.更多还原

【Abstract】 Mice,weighng 22 grams in body weight,were randomisely divided into two groups,40 for each,one control and the other as immunized group.Heat solubilized crude glycoprotein of fish zona pellucida(0.1-0.2mg each), emulsified with Freund adjuvant by routine method,was used for the active immunization of mice by I.M.injection.After three booster injections,the serum antibody titer of immunized mice reached 2~4,five weeks later,it reached 2~3. The estradiol and progesterone contents of mice ovaries and serum of immunized mice were lower than that of the control.Estrous cycles of immuuized mice were prolonged. But,no abnormal changes were observed in the follicular growth,development,maturation and ovulation.The number of ova ovulated of the two groups were practically similar.更多还原