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鲤鱼血红蛋白信息核糖核酸(HbmRNA)的分离
THE ISOLUTION OF RETICULOCYTE MESSAGER RNA FROM CARP
【摘要】 <正> 信息核糖核酸(mRNA)是十分重要的生物活性物质。基因通过转录作用合成mRNA,再由mRNA翻译,编码特定的蛋白质顺序。因此,mRNA是基因和蛋白质之间的桥梁。不仅如此,某些病毒还可以用mRNA反转录编码DNA的程序。一些学者还发现某些高等动物
【Abstract】 This article introduces a method of isolution and purification of reticulocyte messager RNA. For preparating reticulocyte, a new method had been used. The Carp was made anemic by abstrating blood instead of classical method of injecting Phenyhydiazino. Then the total reticulocyte mRNA was prepared using cold phenol. The purified mRNA was used for chromatpgraphy of celloluse Oligo (dT) . Purified mRNA had a typical peak of nucleic acids and biological activity.
- 【文献出处】 海洋科学 ,Marine Sciences , 编辑部邮箱 ,1986年06期
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