【摘要】 <正> 在细胞与原生质体的分离培养与融合的研究中,利用电子显微镜观察高等植物细胞去壁情况、原生质体再生壁的形成过程以及原生质体融合后细胞内细胞器的变化已经是比较普遍采用的手段。但在大型海藻细胞分离与培养方面的研究工作中,利用这一手段进行研究的并不多见。 我们在研究Ⅰ中曾经利用海螺酶解离坛紫菜的营养细胞并成功地培养成紫菜叶状体,但是解离成单离细胞去壁情况如何,当时更多还原

【Abstract】 The ultrastructure of protoplasts of Porphyra haitanensis (Bangiophyceae, Rhodophyta) is studied in this paper. Protoplasts of Porphyra were treated with enzyme mixture for two hours, followed by centrifugation. The enzyme mixture was composed of 1% sea snail digestive enzyme and 0.5% R-10 cellulase in 2 M glucose solution. Fluorescent microscope observation reveals that most of protoplasts obtained show no fluorescent reaction. The protoplasts were collected and fixed for the ultrastructural study. Observation under the electron microscope confirms that protoplasts have only one layer of plasma membrane surrounded without cell wall structure. The ultrastructure of protoplasts is similar to that of vegetative cells.更多还原