【摘要】 作者参照用小鼠胸腺细胞检测白细胞介素-2(IL-2)活性的方法,增加氢化可的松,消除比裂原刺激的干扰,使结果更能表达IL-2的活性,建立了一种简易、快速和稳定的检测方法。采用此法检测了人及小鼠IL-2的活性,经不同样品及同一样品的多次重复试验,效果一致,证明本法重复性好,可作为检测及研究正常或病理情况下IL-2活性变化的手段。更多还原

【Abstract】 In this paper, we describe a new methodfor detecting interleukin-2 （IL-2）.TheConA-activated proliferation of C57BL/6mice thymocytes is extremely sensitiveto the inhibitory effect of hydrocortisone（HC）. and can be completely blocked by avery low concentration of HC(10^-7,mol/L).This inhibition is reversed by theaddition of a 30h PHA-activated humantonsillar lymphocyte supernatants （PHA-SN） or a 24h ConA-activated mousesplenic cell supernatants （ConA-SN）,andthis abrogation of HC-blocked ConA-induced thymocyte activation by PHA-SNor ConA-SN was dose-dependent on thesupernatant concentration,but no thesupernatants containing the product ofLPS-activated marcrophages.The resto-ration of PHA-SN could be completelyremoved by absorption with human T cellblasts,and it was also found that therestoring activity of PHA-SN was greatlydcereased by absorption with human Bcell blasts.These results have stronglysuggested that IL-2 is responsible forthe restoring phenomenon.More than20 experiments demonstrated that thismethod was stable and reproducible.Therefore,it is suitable for research workas well as clinical study.更多还原