【摘要】 在绒毛膜促性腺激素放射受体分析中,作者选用了7％聚乙二醇及0.42％γ-球蛋白(γ-G)0.2毫升分离B、F,其分离结果优于常用的滤膜法及高速离心法,与人血清比较γ-G的分高效果较好,而进口γ-G优于自制品。更多还原

【Abstract】 In the radioreceptor assay,one of thekey steps is the separation of bound radiosubstance(B) and free radio substance (F) atthe end of the reaction.We chose poly-ethylene glycol 6000 (PEG) and γ-globulinprotein for the separation of B and F.Theconcentration of PEG for optimal precipi-tation of receptor bound hCG was 7%(W/V).0.42% of γ-globulin protein gotthe good result of separation.Comparisonwith methods of millipore filter and highspeed centrifuge revealed that the nonspe-cific binding was highest among the threemethods when we used millipore filter.The results were influenced by γ-globulin pro-tein from different sources.The γ-globulinprotein from SIGMA was better than thatprepared by our laboratory.Comparisonbetween γ-globulin protein and serumshowed that with the former,the bindingwas 25.9%, and with the latter 12.6%.Thisis a good method for separation of B and F,because it is convenient and does not needspecific equipment.更多还原