【摘要】 作者对氯丁二烯所致豚鼠肝脏的损害进行了组织化学观察;肝细胞超微结构观察;组织病理学检查及血清生物化学检查。结果提示氯丁二烯所致肝损害的实质是对内质网、线粒体的损害,并影响糖、脂代谢和蛋白质合成,最终导致肝细胞脂肪变性。更多还原

【Abstract】 Guinea pigs were treated with chloro-butadiene,99.9% pure as determined bygas chromatography,by exposure to theconcentration of 37. 52±11. 44, 94.05±26.58,and 340. 22±83. 30mg/m~3, 2h per day for1, 3 and 6 weeks. The results were: 1. Histochemistry study of the livertissue:The amount of glycogen and theactivity of LAP,SDH, G-6PD, HKG,G-6P,5’-N,ICD, MAO decreased;the activity ofLDH and the amount of lipid increased. 2. Study of ultrastructure of the livercells: Changes were mainly hyperplasiaand dilatation of smooth endoplasmicreticulum of liver cells,and rough endo-plasmic reticulum and mitochondria wereinvolved. 3. Histopathology study: The mainchange was massive necrosis. 4. Serum biochemistry study: The ac-tivity of OCT, ICD, GOT, GPT and LDHwas not different from that of the control.In the individual group,albumin wassignificantly different from that in thecontrol group,but the dose-responserelation was not present. The sensitivity of the four types ofstudied marker decreased according to theabove order. The essence of liver toxicity by chloro-butadiene could be summarized as follows:injury to endoplasmic reticulum andmitochondria,disturbance of glycogenand lipid metabolism and protein synthesis,interference of mitochondrial oxidativephosphorylation and citric cycle,anda final induction of liver cell fattydegeneration.更多还原