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钩端螺旋体病人血清抗体的SPA-ELISA检测法

SPA-ELISA IN DETECTION OF LEPTOSPIRAL ANTIBODIES IN SERA OF LEPTOSPIROSIS PATIENTS

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【作者】 叶丹霓汪成孝阎蓉华赵蜀岷戴保民

【Author】 Ye Danni;Wang Chengxiao;Yan Ronghua;Zhao Shumin;Dai Baomin Research Unit of Leptospirosis

【机构】 华西医科大学钩端螺旋体病研究室华西医科大学钩端螺旋体病研究室

【摘要】 用酶标葡萄球菌A 蛋白作ELISA 检测临床确诊、显凝试验为阳性的钩端螺旋体病人血清59份,同时用40份健康人血清与酶标抗体作ELISA进行比较。两种方法都有较高的敏感性和特异性,而酶标葡萄球菌A 蛋白具有制备简单,标记步骤简便,比较稳定和易被推广等优点,可替代酶标抗体在ELISA中应用。

【Abstract】 SPA-ELISA was used to detect lepto-spiral antibodids in leptospirosis patients.Fifty-nine serum samples of proved patientswere examined by SPA-ELISA and sheepanti-human IgG-ELISA. The results showedthat the detection rates of the specific anti-body were 98.30%and 93.22% respectively;while in the control sera of normal persons,all were negative. The results of the detec-tion indicate that both SPA-ELISA andsheep anti-human IgG-ELISA are sensitiveand specific, but SPA-ELISA is a simple,stable and available method in the clinicalpractice.

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