【摘要】 <正> 葡萄糖氧化酶催化葡萄糖被水中的溶解氧所氧化,生成葡萄糖酸和过氧化氢。生成的过氧化氢再在过氧化物酶的存在下将原来无色的邻联二茴香胺氧化成有色产物的反应是:更多还原

【Abstract】 In the coupled reaction of glucose oxidaseperoxidase-o-dianisidine system, the aerobic oxidation of glucose to gluconic acid and hydrogen peroxide takes place. In the presence of horseradish peroxidase, the rapid oxidation of odianisidine by hydrogen peroxide can be followed. The product is determined spectrophotometrically at 440 nm. This enzymatic method was used for the determination of glucose in blood, and Ag⁺, Hg²⁺, VO₃^-as inhibitors can also be determined.更多还原