【摘要】 本文报告了一种乙型肝炎全抗原亚型疫菌,该疫苗不去除Dane颗粒,它是用HBsAg和HBeAg阳性携带者血清。并且为adr。adw及ayw三种亚型。分别经聚乙二醇沉淀和超速离心纯化。产物在电镜下除HBsag外可见到Dane颗粒,然后除菌、甲醛灭活。稀释并加入氢氧化铝佐剂制成的,每种亚型疫苗分别多部位(脚掌、腹服沟、颌下等),皮下接种豚鼠每次总量1ml,三次免疫后。全部动物均产生抗—HBs。抗—HBc每批动物仅个别阳性。抗—HBe全部阴性,但必须考虑豚鼠对HBV三个抗原系统免疫应答灵敏性远较人类和黑猩猩差,作为全抗原乙肝疫苗研制、本文进行了初步尝试。更多还原

【Abstract】 In this paper a whole antigen vaccine of HBV is reported. The vaccine does not exclude Dane particles, It was derived from the sera of the carriers who were both HBsAg and HBeAg positive, and was of three subtypes i. e. adr adw, and ayw. The HBV was purifed with, PEG precipitation and was ultracentrifuge. Besides HBs Ag, Dane particles were found under electron microscopy in the product Then bacteria were removed, and the product was inactivated with formalin, and diluted and Al （OH）₃ adjuvants were added to it Hypodermic inoculation with each vaccine was done at. the submaxilla, inguen, back and sole of the guinea pigs The total amount of inoculation was 1 ml per animal. After being inooulted three times, anti-HBs occurred in all animals. Anti-HBc was positive in only a few in each group, w1hile anti-HBc was negtive in all animals. But it must be noticed that the immunoresponse of guinea pig is lowar than that of human and chimpanzee: As a study of whole antigen HBV vaccine only a preliminary research has been carried out.更多还原