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间接免疫荧光法检测婴幼儿急性下呼吸道合胞病毒感染的特异性IgM

A STUDY FOR DETECTION OF SPECIFIC IgM ANTIBODY AGAINST RSV IN INFANTS AND YOUNG CHILDREN BY IIFA

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【作者】 严淑芳易著文高洁生曾东源杨艳辉周汉昭熊正东陈英杰

【Author】 Yan Shufang Yi Zhuwen Gao Jisesheng Zeng Dongyuan Yang YanhuiVirological Laboratory of Pediatrics, The Second Affiliated Hospital, Hunan Medical CollegeZhou Hanzhao Xiong Zhengdong Chen YingjieDepartment of Pediatrics, The Second Affiliated Hospital, Hunan Medical College

【机构】 湖南医学院第二附属医院儿科病毒室湖南医学院第二附属医院儿科教研室湖南医学院第二附属医院儿科教研室

【摘要】 本文对1984年1~3月和1985年3~8月共107例急性下呼吸道感染的住院患儿,采用间接免疫荧光法检测其急性期血清抗RSV特异性IgM抗体,并与病毒分离和/或中和试验比较,敏感性为82.1%,特异性为71.8%。RSV感染患儿发病后3天内大多数病例即可从血清中检测出RSV-IgM,因此该法具有早期诊断价值。

【Abstract】 RSV-IgM has been detected with IIFA in 107 cases of ALRTI infants and younger children. As compared with virus isolation and/or neutralization test, the sensitivity of IIPA was 82.1%, the specificity 71.8%. RSV-IgM of cases was detected within the first 3 days. It is suggested that the detection of RSV-IgM of ALRTI is satisfactory method for early diagnosis of RSV infection.

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