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厌氧菌培养的四种送检法对比研究

COMPARATIVE STUDY OF ANAEROBIC BACTERIAL CULTURE IN FOUR TANSPORT SYSTEMS

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【作者】 唐银; 谢振英; 郭思健;

【Author】 Tang Yin Xie Zhenying Guo Sijian Department of Clinical Laboratory, The First Affiliated Hospital, Hunan Medical College

【机构】 湖南医学院第一附属医院检验科; 湖南医学院第一附属医院检验科;

【摘要】 采用液体培养基管、半固体琼脂培养基管、无菌干空针与无菌干空管等方法同时送检厌氧菌培养标本;以无菌干空管法作对照,对比检测了四种方法的检出率。结果表明,半固体琼脂培养基管送检法阳性率最高,且方法简便,有实用值价。

【Abstract】 Three transport systems are introduced, the liquid and semisolid agar medium tubes and the sterilized dry aerobic syringe, comparing with that of the sterilized dry aerobic tube for culture of anaerobic bacteria. The positive rate of the sterilized dry aerobic tube was 6.3% liquid medium tube 31.3%, semisolid medium tube 46.9%, and the sterilized dry aerobic syringe 34.9%. The authors adopted the semisolid medium tube to collect various specimens. In an examination of 202 specimens, the positive rates of the pus, the ascitic fluid and the bile were 79%, 72% and 62% respectively. It is preferable in clinical practice.

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