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金黄色葡萄球菌噬菌体增殖和分型法的探讨

AN INVESTIGATION ON THE PROPAGATION OF BACTERIOPHAGE AND BACTERIOPHAGE TYPING OF STAPHYLOCOCCUS AUREUS

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【作者】 徐秀华; 翁绍纪; 张铮; 熊宏恩; 何泗哲;

【Author】 Xu Xiuhua Weng Shaoji Zhang Zheng Xiong Hong-en He SizheDepartment of Infectious Diseases,The First Affiliated Hospital,Hunan Medical College

【机构】 湖南医学院第一附属医院传染病科; 湖南医学院第一附属医院传染病科;

【摘要】 以伦敦中央公共卫生实验室提供的23株金黄色葡萄球菌的基础分型噬菌体,对在医院内外收集的449株金黄色葡萄球菌进行了分型。对噬菌体的增殖和分型方法加以改良,使449株金黄色葡萄球菌的噬菌体分型率达91.54%。本文介绍噬菌体逆向分型法,认为此法可提高分型率,并能发现金黄色葡萄球菌的新型菌株。

【Abstract】 Four hundred and forty-nine strains of staphylococcus aureus were collected from our hospital and the community. They were typed by an international basic set of typing phages prepared by the Central Public Health Laboratory Service, Colindale, London. we have improved the method for propagating the phage and raised the rate of typability of S. aureus to 91. 54%. The method of reverse phage-typing is recommended, because not only does it increase the rate of typing but also it may be helpful in discovering new strains of S. aureus.

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