【摘要】 <正> 在神经生理、针麻原理等学科研究中,高比度的氚标记多巴胺是一种常用的重要示踪剂。关于它的制备方法可分为气液交换法和化学合成法两种。我们采用化学合成法制得(7,8~3H)多巴胺,反应式为更多还原

【Abstract】 Dopamine is a neurotransmitter in the central nervous system. (7,8-3H) dopamine is an important tracer for the study of physiological functions and metabolic processes. It was prepared by catalytic reduction of 3-hydroxy-4-methoxy-8-nitro-styrene with tritium gas. At the end of reaction, hydrobromic acid was added and heated to remove the methoxyl group. The crude product was purified by paper chromatography. The purity of (7,8-3H) dopamine was identified by IR, UV, PC and 3H-NMR spectra. The radiochemical purity was over 95% and the specific activity was 1.26 × 1012Bq/mmol (34Ci/mmol). The distribution of labelled tritium in molecule was shown as follows: 55.4% at position 7 and 44.6% at position 8.更多还原