【摘要】 <正> 环磷酸鸟苷(cGMP)和环磷酸腺苷组成一对矛盾。它们对细胞的生长、繁殖及生理过程起着重要的调节作用,在中医药基础理论及临床研究中日益受到重视。但是cGMP在体液及组织中含量甚微,因此需要有一个灵敏、简易、特异的测定方法。更多还原

【Abstract】 This paper describes iodine labelled radioimmunoassay for the cyclic GMP. Preparations of antigen, antibody and radioligand are described. A second antibody separation of simple high sensitivity method for the assay of cyclic GMP is presented.The cross-reactivity of this eyelid GMP antiserum with cyclic AMP and GTP are 0.008% and 3.1×10-3% respectively.Sensitivity of the method is 1.5 fmol cyclic GMP per tube. Recovery of the method is 104.0 ± 4.7% and variety coefficient in batch is 2.8%.Cyclic GMP content in the plasma of normal man is 4.60 ±0.70 pmol/ml.更多还原