This paper presents a method on improving Cerenkov radiation counting efficiency for 32P, 131I, 45Ca, 35S and 14C radionuclides by using the emulsifier and wavelength shifter mixed solution as medium. The use of this mixed-medium for Cerenkov counting of high-energy β emitters is able to increase the counting efficiency 40% to 50%as compared to the water medium, and even the low-energy β emitters which below the Cerenkov threshold can also obtain higher counting efficiency. It is found that this method show...