【摘要】 制备了多种类型的脂质体,用化学发光法去测量其上的脂膜过氧化和自由基反应。试验表明:脂质体脂膜的过氧化与介质的温度、pH、pO₂及脂质体的浓度有关,试验过程中应该控制这些因素。用细胞色素C和H₂O₂诱发扩增化学发光时,还应控制反应的时间。脂质体接近于生物膜,是研究脂质过氧化的好材料。用化学发光法测量脂质过氧化和自由基反应,具有灵敏、快速、简便的优点。更多还原

【Abstract】 A model of measuring lipid membrane peroxidation and free radical reaction is established by chemiluminescence（OL）of liposomes.The extent of lipid membrane peroxidation of liposome depends on the temperature,pH,pO₂（oxygen partial pressure）of the medium and its liposomal concentration.These factors must be precisely controlled throughout the experiment.When cytochrome C and H₂O₂ are used to enhance CL,the length of reaction time should also be carefully controlled.As liposomes are similar to biological membranes in many aspects,naturally,it becomes a good material for investigating lipid membrane peroxidation.For study of lipid peroxidation,OL measurement seems to be a faster and simpler method with higher sensitivity.更多还原