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萤光分光度法测定乙烯基吡咯烷酮—醋酸乙烯酯共聚物组成
Determination of the Components of Vinylpyrrolidone-vinylacetate Copolymer by Fluorescence Spectrophotometry
【摘要】 本文研究以1—苯胺基—8—萘磺酸(ANS)作萤光标记测定乙烯基吡咯烷酮—醋酸乙烯酯共聚物组成。测定条件是;ANS浓度为10-5mol/1、共聚物浓度为0.1g/1~0.01g/1,以365nm光激发,溶液为:pH=5.71的磷酸盐缓冲液,测定萤光强度。用校正曲线法测定共聚物组成(乙烯基吡咯烷酮重量百分比由50%~100%)。该方法灵敏度为:6×10-3g/1,相对误差小于6%。
【Abstract】 A fluorescence spectrophotometric determination of the components of vinylpyrrplidone-vinylacetate copolymer is described. 1-anilino-8-naphthalenesulfonic acid is added at a concentration of 10-5M/L to serum as the standard. Analytical conditions are given as follows:pH = 5.59; wavelength of exciting source is 365nm with a detection limit of 6ppm. Calibration curve is applied for the measurement ( weight percentages ranging from 50% to 100%).
- 【文献出处】 河北大学学报(自然科学版) ,Journal of Hebei University(Natural Science Edition) , 编辑部邮箱 ,1986年03期
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