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家兔实验性切创的酶组织化学观察

ENZYME HISTOCHEMICAL STUDY OF EXPERIMENTAL RABBIT INCISED WOUND

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【作者】 刘世沧; 廖志钢;

【Author】 Liu Shicang Liao Zhigang Department of Forensic Pathology Facttlty of Forensic Medicine West China University of Medical Sciences

【机构】 华西医科大学法医系法医病理学教研室; 华西医科大学法医系法医病理学教研室;

【摘要】 本文报告30只家兔实验性切创在伤后1至12小时创壁的酶组织化学反应。LDH,SDH,NADH,α-GPDH 四种酶反应阳性。创壁酶活性增高的原因部分是局部原来的纤维细胞物质代谢升高,部分是由于急性炎细胞在伤区积聚。作者认为伤后1小时创壁酶反应阳性有助于确定生前伤,并推荐用 PTAH 染色确诊早期创面的微量纤维素性渗出。

【Abstract】 Enzyme-histochemical investigations on experimental incised injury of thirty rabbits fortiming of wound were reported.In peripheral areas of wounds,strong reaction of six kinds ofdehydratase(ACP.AKP,ATP.ANAE.β—GA,β—Gr).were demonstrated 1~2 hrs followingincision while no reaction of four kinds of dehydrogenase(LDH.SDH.NADH.α—GPDH)werefound.The activity of dehydratase are explained by the enhanced metabolism of fibrocytes and theaggregation of acute inflammatory cells.The author suggests that the positive enzyme histochemicalreactions indicate the vital reaction in the first two hours,and PTAH staining is helpful for thediagnosis of micro—exudation of fibrin in the early stage of injury

  • 【文献出处】 中国法医学杂志 ,Chinese Journal of Forensic Medicine , 编辑部邮箱 ,1986年01期
  • 【被引频次】4
  • 【下载频次】36
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