【摘要】 应用巴比妥缓冲液琼脂糖凝胶电泳法测定血清LDH同工酶常出现LDH₅区带向负极移动,落在原点之后,同时LDH_4-5区带含量常偏低。为了纠正上述缺点,我们根据日本长岭光隆等提出的焦磷酸缓冲液琼脂糖电泳法进行测定与方法学探讨。我们对100例正常血清标本用本法与巴比妥法同时测定LDH同工酶的百分含量,本法测定结果;LDH₁ 26.5±4.3,LDH₂ 33.1±3.7,LDH₃ 22.3±3.5,LDH₄ 12.0±2.8,LDH₅ 6.1±2.9,而巴比妥法测定结果:LDH₁ 29.6±4.5,LDH₂ 35.0±5.8,LDH₃ 21.5±4.4,LDH₄ 9.3±4.1,LDH₅ 4.6±2.9。比较二法测定结果表明,本法对LDH₄和LDH₅区带较为灵敏,p值分别为P<0.05和P<0.01,有显著性差异,其次,本法在一定条件下,具有使电泳各区带推向沟前的良好效果,这是本法优於巴比妥法之处。在方法学上,我们对使用载体、冷却装置及固定液、洗脱等步骤均进行某些改进,使本法更为简便、更适合于临床应用,且便于应用光密度扫描。更多还原

【Abstract】 The serum LDH isoenzyme percentages were determined in 100 normal persons by the electrophorisis of agarose gel of pyrophosphoric acid buffer solution. The X± SD of LDH₁ was 26. 5±4. 3, LDH2 33. 1±2. 7, LDH₃ 22. 3± 3. 5, LDH₄ 12. 0±2. 8 and LDH₅ 6. 1±2. 9, the results were compared with the eleetrophoresis of agarose gel of barbiturate buffer solution, we found that the LDH₄ and LDH₅ band of this method was more sensitive than the barbiturate method, P value was of statistical difference（p<0. 05 and p<0. 01）. This method had a good result that made all electrophoric band to depart from the original point toward the anode and to be scanned more easily: we also made some other improvement in methodology, therefore, the method is recommended for use in clinical pratice.更多还原