【摘要】 对以水稻幼穗为外植体诱导的愈伤组织进行振荡培养,分离出单细胞。观察到两种细胞类型:一类细胞呈圆形,其核较大,细胞质较致密,分裂活性较强;另一类细胞呈长形或不规则形,其核较小,细胞质较少,很少见到分裂现象。这两类细胞数目与培养基中大量元素、肌醇和2.4-D浓度有很密切的关系。通过半连续悬浮培养使圆形细胞增加,培养40～60天后圆形细胞数达95%。观察到单细胞活体连续分裂的过程及从悬浮培养细胞液中滤出的单细胞再生成了植株。更多还原

【Abstract】 Single cells were isolated from shaking cultures of calli induced from explants of young panicles of the rice plant. Two types of cells were observed, one was round in shape with larger nucleus and dense cytoplasm and engaged in active cell division, the other was elongated or irregular in shape with inconspicuous nucleus and cytoplasm and seldom divided. The number of the two types of cells varied according to the concentration of macro-and micronutrients, myo-inositol and 2.4-D in the culture medium. The number of round cells increased during the passages, up to 95% after 40～60 day at the passage. Successive divisions from single cells were observed and plantlets have been regenerated from single cells originating from the suspension culture.更多还原