【摘要】 应用高压电镜放射自显影术研究腹毛类无小核伪尖毛虫和Styx sp.大核DNA合成。证明大核DNA合成发生于复制带的后区。小核DNA合成发生在小核有丝分裂的早前期(当大核DNA合成处于最后阶段时),高压电镜最大的优点是允许使用厚切片和连续厚切片。厚切片含有比超薄切片较多的放射性,因此大大缩短对核乳胶的曝光时间。更多还原

【Abstract】 The synthesis of DNA in hypotrichous ciliates, amicronucleated strain of Oxytricha sp.and Styx sp. was studied by high voltage (1000kV) electron microscope autoradiography. The main advantage of high voltage electron microscopy is that it allows the use of thick sections as well as thick serial sections, which has markedly shorten the autoradiographic exposure time. The autoradiographs show that DNA synthesis occurs in the rear zone of a replication band in the macronucleus. Micronuclear DNA synthesis in Styx sp. takes place in early prophaseat a time when macronuclear DNA synthesis is in its terminal Phase.更多还原