【摘要】 本文应用冷冻复型电镜技术对豚鼠肝脏进行了观察。发现肝细胞不同部位的细胞膜有所差异。在肝细胞的狄氏腔面和微胆管腔面的微绒毛,其PF面上的膜内微粒较其余部PF上的多而密。紧密连接只存在于微胆管的四周。缝管连接只见于两肝细胞相邻面。肝细胞核的核孔每μm~2平均有12.8个。粗面内质网上未见核孔样结构,而高尔基复合体的扁平囊上则有。滑面内质网多有分支交织成网。线粒体很多。肝血窦内皮的筛板部有成群分布、大小不一的内皮孔,平均直径为105nm,密集处每μm~2有25个;在较厚的内皮细胞突上无孔,但有饮液泡及贯穿胞质的小管。内皮外无基膜,但可见内皮边缘部相互重叠,二者间有很窄的间隙。肝血窦腔内可见枯氏细胞的胞体及其长突附于内皮细胞上,胞体内除有大小不一的泡和小管状结构外,还可见有大小不等的胞膜凹陷与之相通。以上各种细胞膜PF面上的膜内微粒都远较EF面上为多,相对而言肝细胞的较枯氏细胞和内皮细胞的为多。本文根据观察结果与文献中其它动物肝脏和用其它方法对肝脏的电镜观察进行了对比和讨论。更多还原

【Abstract】 The guinea pig liver was studied by the method of freeze-fracture replica electron microscopy. The results were as follows: 1. The cell membrane of various parts of the liver cell varies with each other, the intramembranous particles on the pF of the microvilli protruding from the liver cell to the Disse’s space and bile canaliculi are more than other parts;tight junctions only present around the bile canaliculi, and gap junctions may only beobservedon the appositional surfaces of adjacent liver cells. 2. The mean number of the nuclear pores on the nuclear membrane of liver cell is 12.8 per μm~2. 3. Nuclear pore like structure is not present on the rough endoplasmic reticulum, but may be revealed on the cisterna of Golgi complex. 4. The endothelial pores are only present on the sieve plates, while pinocytotic vesicles occur in the cytoplastic processes and the mean diameter of the pores is 105nm, the number of it is 25 per μm~2; there is no basement membrane out side of the endothelium, yet overlappings of the marginal parts of adjoining endothelial cells may be seen and a narrow slit is present between them. Within the lumen of the sinusoid, Kupffer cells with long processes may be revealed to adhere the endothelium but with out junctional formation, various vesicular and warm like (?)bular structures are plentiful, and some of them may be communicated with pinocytotic openings. 5. Intramembranous particles on the PF of various cell membranes are much more than those on the EF, and it is relatively more numerous on the liver cell than other cell types.更多还原