【摘要】 <正> 在小麦的组织培养中,研究了影响叶片愈伤组织诱导和分化频率的某些因素。试验结果表明:在较高温度下生长的幼苗,未伸出叶片或半伸出叶片基部的片段,愈伤组织的诱导和分化频率较高;不同品种的叶片,愈伤组织的诱导和分化频率有很大差异;直径大于5毫米,一月龄的愈伤组织,分化根的频率较高:补加激素IAA1.0毫克/升和KT1-2毫克/升的培养基,明显促进幼苗的分化。更多还原

【Abstract】 A study of sertain factors, affecting the frequencies of induction and differentiation of callus in tissue cultures of wheat leaves has been carried out. The results are summarized as follows: (1) More segments induced callus in basal region of the unextruded leaf or the youngest extruded leaf of plants grown under higher temperature. (2) It’s much differentil for the frequencies of induction and differentiation of callus from leaves of different wheat variety. (3) It’s higher for the frequency of root differentiation from the callus of the diameter over 5mm and the age of one month. (4) The medium supplemented with 1.0mg/1 of IAA and 1.0-2.0mg/1 of KT obviously promoted the bud differentiation.更多还原