【摘要】 作者用透射电镜研究了四川南坪疫区利什曼病犬肝、脾内无鞭毛体的超微结构。观察到其膜下微管数为81—135,平均101±10。微管平均直径和微管间距分别为31±8.6nm和28±0.65nm。对比文献资料,与从貉和美国Oklahoma犬等动物体内获得的无鞭毛体膜下微管数有明显不同;而与观察人体利什曼原虫所获结果相近。为进一步研究人体利什曼病与病犬的关系提供了线索。研究还发现病犬前肢、耳、鼻皮肤内无鞭毛体数量较多;内脏中以颈淋巴结内数量最多,其次为肝、脾,以下依次为骨髓、胰、睾丸、附睾。肺内仅遇见原虫。由于前肢皮肤原虫数量(3616.3±174.6)可高出骨髓(397±37.7)近10倍,为自多处皮肤取材用于诊断提供了客观依据。另外,首次从病犬延髓印片中查见无鞭毛体,提示其侵犯中枢神经系统的危害性是存在的。更多还原

【Abstract】 Ultrastructural studies of amastigotes have shown that the numbers of subpel-hoular microtubules may be useful morphological parameters for differentiating Lei- shmania spp. This electron microscopy study was used to help classify and to obtain basic biological data about the leishmania donovani amastigotes from an infected dog from the endemic focus. The number and the diameter of subpellicular microtubules (St) and the St intervals of 48 amastigotes observed by electron microscopy were 101±10, 31 ± 8.6nm and 28 ± 0.65nm, respectively. Nucleus, nuc-leoid, kinetoplast, mitochondrium, flagellar pocket, etc. were also observed. The St number of leishmanial amastigotes reported in this study was quite different from that of amastigotes isolated from recoon dog (Xu et al, 1984) or from the dog from Oklahoma, USA (Kocan, K. M. et al, 1983) . Moreover, the amastigotes distributed in the skin of forelegs (3616. 3±1 74.6) , ear (3452 ± 237.7) and nose (2579.3 ± 338.7) of the dog were much more than those in the other part of the skin and organs. It was also found that the amastigotes in cervical lymphnodes (2426 ± 80.2), liver (1915.3 ± 95.6) and spleen (1566 ± 247.9) have the greatest number among the organs examined. In marrow, pancreas, testis, epididymis, etc. , the amastigotes were in turn much less than those in skin or liver and spleen. Since the number of amastigotes in the skin of forelegs was as nearly 10 times as that in marrow, detection of amastigotes in the skin from different part of infected dog may be helpful for the diagnosis. Furthermore, the first discovery of the amastigotes in medulla oblongata suggests the presence of perniciousness of this protozoal invasion in the central nervous system.更多还原