【摘要】 <正> 引言 随着昆虫病毒的应用不断扩大也必须从保护坏境,保护人畜健康出发,进行严格试验,以确定其安全。本试验用舞毒蛾NPV对脊椎动物小白鼠、豚鼠、家兔、家鸽、金鱼,作了安全试验,结果未见病变和病毒增殖。为了进一步检查舞毒蛾NPV是否能在脊椎动物和人体外培养的细胞中复制或引起病变,以便从细胞水平确定舞毒蛾NPV的更多还原

【Abstract】 In this experiment, several vertebrates, including mice, guinea pigs, domestic rabbits, pigeons and golden fish, were artificially infected with NPV of Gypsy moth in order to test its safety ,to vertebrates, and no pathological symptom and virus amplification was discovered. In order to test whether this virus is capable of replicating and causing pathological symptom in vertebrates and men’s cells in vitro, the cultured cells of donkey’s lungs, sheep’s kidneys, monkey’s kidneys, chick’s embryo and amnion and men’s kidneys were also artificially infected and then examined under light and electron microscopes. No pathological symptom and virus amplification were found. Therefore, the result of this experiment indicates that the NPV of Gypsy-moth is safe to men and animals tested in the experiment.更多还原