【摘要】 本文用流行性出血热病毒单克隆抗体致敏羊血球,做反向被动血凝和被动血凝抑制试验。检查出血热病毒抗原和抗体,不仅敏感,性能稳定,而且操作简便、快速。更多还原

【Abstract】 Reverse passive hemagglutination(RPHA) and reverse passive hemagglu tination inhibition(RPHI) test were carried out by using monoclonal anti-body 25 1 sensitized red blood cell(M-RBC) to detect the antigen and anti -body of epidemic hemorrhagic fever virus(EHFV). The comparative results of RPHA and hemagglutination test(HA) of EHFV showed that RPHA was more sensitive and simpler than HA. M-RBC can be agglutinated by the antigen of EHFV either prepared by sucrose acetone method or saline extract method, but for HA the goose RBC can only be agglutinated by the antigen prepared by sucrose-acetone method. The titer of RPHA is on the average 2-4 times higher than HA. We also found that antigen for RPHA is better when low speed centrifugation (500 rpm/min) is used than high speed (10,000 rpm/min). We also compared the antibody titers of EHF pati ents by RPHI and IF method. The same positive rate was found by both method in 25 sera. RPHI was easier to carry out than IF. The M-RBC is stable at 37℃ for 7 days and at 4℃ for more than 90 days. The result can be read in 60 minutes.更多还原