【摘要】 用IgM-ELISA法检测115份不同病日的流行性出血热（EHF）患者血清,结果表明,发病5天内可测出EHF特异性IgM抗体,阳性率达87.5％,最高滴度达10^?,本法特异性好,敏感性高于ELISA竞争法,不需特殊设备,与免疫荧光法相比,更适合基层推广使用。更多还原

【Abstract】 Viral IgM antibodies was demonstrated in 115 sera from 58 patients with epidemic hemorrhagic fever ( EHF ) by using an IgM-ELISA with anti-μ coated solid phase. The results obtained showed that the IgM antibodies to EHF virus ( EHFV ) coubld be detected within day 5 after onset of illness and that the antibody titers revealed a peak by day 10 with a highest titer of 10-6. The percentage of EHFV IgM-positive sera accouned for 87.5% within day 5 and 100% by day 10, then decreased rapidly,and turned negative after day 60. Experimental results indicate that this IgM-ELISA technique successfully eliminated nonspecific reactions, including the false positive reactions induced by the rheumatoid factor.As compared with the indirect immunofluorescence test and the competitive ELISA, this technique was more sensitive. Taken together, the technique is not only sensitive and specific, but also simple and rapid and thus may be applied for the early diagnosis of EHF.更多还原