【摘要】 <正> 鸭瘟病毒(DPV)是一种禽疱疹病毒,引起鸭急性致死性传染病。本病毒自1923年报道后,半个多世纪以来,对其生物学特性等虽有某些研究,但有关分子病毒学研究则国内外尚无报道。本研究通过对DPV DNA的限制性内切酶图谱的分析,为进一步开展DPV的分子病毒学研究提供基础资料,并对DPV的分类地位作些探讨。更多还原

【Abstract】 Endonuclease maps of an attenuated duck plague virus ( DPV ) were reported.DPV was replicated in CEF culture.The harvested virus was concentrated by differential precipitation with ammonium sulphate and purified through 25-45%(w/v ) sodium potassium tartrate gradiants.The DPV DNA was isolated by means of protease digestion-SDS-phenol method before cleavage with restriction endonuclease EcoR Ⅰ,BamHI,Hind Ⅲ and Pst Ⅰ respectively. The DNA fragments ware resolved by 0.7% agarose gel electrophoresis. The molecular weight of DPV genome, estimated by summation of fragment sizes from cleavage patterns, ranged from 91.84×106 to 94.84×108. In addition, by isopycnic CsCl gradient centrifugation, the buoyant density of DPV particles also was determined as 1.272g/ml. The classification of DPV was discussed.更多还原